Buffers & Reagents

Using the most stringent definition, a buffer is simply a solution used to prevent sudden changes in pH. However, in the biological sciences, the term buffer has come to mean much more. Most experimental biology involves manipulating a set of chemical reactions that underpin a biological state in vitro, and observing the outcome. All life is a synthesis of complex chemistry. As with any reaction, a given set of environmental conditions must be met before a reaction can occur. Commercial buffers for use in the life-sciences contain specific chemicals that facilitate a desired chemical reaction (e.g. enzyme cofactors, protein stabilizers, or carrier proteins). A buffer must be carefully chosen for a given application, based on individual requirements & criteria.

At genomics-online you will find molecular biology grade agarose as well, ideally suited for routine analysis of DNA or RNA by gel electrophoresis and blotting. The agarose can be purchased in convenient AgarTabs - provided as a tablet format (0,5g/tablet), you avoid tiresome weighing and moreover contamination during weighing. In order to stain the DNA or RNA to visualize them in agarose and polyacrylamide gels we offer several nucleic acid stains. The dyes eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.

Browse the products to at genomics-online to find high-quality Taq buffers, molecular biology grade agarose, dyes, restriction enzymes, reaction buffers and many more products. For further questions regarding our products, please contact our customer service via phone, live chat, or email.

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SafeView™ Classic is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.

Application:
SDS-PAGE, Agarose Gel Electrophoresis
Storage:
4 °C
Publications
  • Doh, Kim, Oh, Lee: "Identification and monitoring of Korean medicines derived from Cinnamomum spp. by using ITS and DNA marker." in: Genes & genomics, Vol. 39, Issue 1, pp. 101-109, 2017 (Pubmed)
  • Dudek, Gossan, Yang, Im, Ruckshanthi, Yoshitane, Li, Jin, Wang, Boudiffa, Bellantuono, Fukada, Boot-Handford, Meng: "The chondrocyte clock gene Bmal1 controls cartilage homeostasis and integrity." in: The Journal of clinical investigation, Vol. 126, Issue 1, pp. 365-76, 2016 (Pubmed)
  • Do, Kim, Yang, Pyo, Kim, Lee, Do, Kim, Chae, Sohn: "Decreased expression of p27 is associated with malignant transformation and extrathyroidal extension in papillary thyroid carcinoma." in: Tumour biology, Vol. 37, Issue 3, pp. 3359-64, 2016 (Pubmed)
  • Zhao, Tsao, Lee, Tsai, Wang, Chuang, Wu, Lin: "Optical fiber sensor based on surface plasmon resonance for rapid detection of avian influenza virus subtype H6: Initial studies." in: Journal of virological methods, Vol. 233, Issue , pp. 15-22, 2016 (Pubmed)
  • Zu, Tan, Ren, Ying: "Facile and phase-defined determination of HLA alleles with morpholino-functionalized nanoparticle probes." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. , Issue , pp. , 2016 (Pubmed)
  • Mizan, Jahid, Kim, Lee, Kim, Ha et al.: "Variability in biofilm formation correlates with hydrophobicity and quorum sensing among Vibrio parahaemolyticus isolates from food contact surfaces and the distribution of the genes involved in ..." in: Biofouling, Vol. 32, Issue 4, pp. 497-509, 2016 (Pubmed)
  • Vivero, Jaramillo, Cadavid-Restrepo, Soto, Herrera: "Structural differences in gut bacteria communities in developmental stages of natural populations of Lutzomyia evansi from Colombia's Caribbean coast." in: Parasites & vectors, Vol. 9, Issue , pp. 496, 2016 (Pubmed)
  • Anjali, Ganga, Nadiya, Shefeek, Sabu: "Intraspecific variations in cardamom (Elettaria cardamomum Maton): assessment of genomic diversity by flow cytometry, cytological studies and ISSR analysis." in: SpringerPlus, Vol. 5, Issue 1, pp. 1560, 2016 (Pubmed)
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Catalog No. ABIN4219136
$64.17
1 mL
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Safe-Red™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.

Application:
SDS-PAGE, Agarose Gel Electrophoresis
Storage:
4 °C
Publications
  • Vlasschaert, Xia, Gray: "Selection preserves Ubiquitin Specific Protease 4 alternative exon skipping in therian mammals." in: Scientific reports, Vol. 6, Issue , pp. 20039, 2016 (Pubmed)
Catalog No. ABIN4219139
$64.17
1 mL
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Application:
Polymerase Chain Reaction
Concentration:
10 X
Storage:
-20 °C
Publications
  • Koo, Mojib, Thacker, Bej: "Comparative analysis of bacterial community-metagenomics in coastal Gulf of Mexico sediment microcosms following exposure to Macondo oil (MC252)." in: Antonie van Leeuwenhoek, Vol. 106, Issue 5, pp. 993-1009, 2014 (Pubmed)
  • Faber-Hammond, Phillips, Park: "The sockeye salmon neo-Y chromosome is a fusion between linkage groups orthologous to the coho Y chromosome and the long arm of rainbow trout chromosome 2." in: Cytogenetic and genome research, Vol. 136, Issue 1, pp. 69-74, 2012 (Pubmed)
  • Tonikian, Sidhu: "Selecting and purifying autonomous human variable heavy (VH) domains." in: Methods in molecular biology (Clifton, N.J.), Vol. 911, Issue , pp. 327-53, 2012 (Pubmed)
Catalog No. ABIN1536551
$10.00
1.5 mL
Plus shipping costs $45.00 Will be delivered in 2 to 3 Business Days
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Application:
Positive Control
Concentration:
1 mg/mL
Storage:
4 °C/-20 °C
Catalog No. ABIN2452214
$111.56
30 μg
Plus shipping costs $45.00 Will be delivered in 5 to 6 Business Days
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Application:
cDNA Synthesis
Catalog No. ABIN920950
$55.00
5 mL
Plus shipping costs $45.00 Will be delivered in 4 to 6 Business Days
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SafeView™ FireRed is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.

Application:
SDS-PAGE, Agarose Gel Electrophoresis
Catalog No. ABIN4219142
$58.06
1 mL
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Safe-Green™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.

Application:
SDS-PAGE, Agarose Gel Electrophoresis
Catalog No. ABIN4219137
$64.17
1 mL
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Safe-White™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.

Application:
SDS-PAGE, Agarose Gel Electrophoresis
Catalog No. ABIN4219140
$64.17
1 mL
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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SafeView Plus™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.

Application:
SDS-PAGE, Agarose Gel Electrophoresis
Catalog No. ABIN4219141
$100.83
1 mL
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Safe-Pack™ dyes represent a new and safe nucleic acid stains for the visualization of nucleic acids in agarose and polyacrylamide gels. These dyes eliminate the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.

Application:
SDS-PAGE, Agarose Gel Electrophoresis
Components:
SafeView™ contains 1 mL each of Safe-Green™, Safe-Red™ and Safe-White™.
Storage:
4 °C
Catalog No. ABIN4219138
$119.17
1 set
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Anti-Taq Antibody is the ultimate solution to eliminate non-specific amplification.

Application:
Polymerase Chain Reaction
Catalog No. ABIN4219143
$143.61
250 U
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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GC Enhancer can be used to increase sensitivity and specificity for GC/AT-rich template or complex template.

Application:
Polymerase Chain Reaction
Catalog No. ABIN5519393
$12.00
200 μL
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Application:
Agarose Gel Electrophoresis
Concentration:
6 X
Catalog No. ABIN1536496
$48.00
5 mL
Plus shipping costs $45.00 Will be delivered in 9 to 11 Business Days
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Application:
DNA Separation, RNA Separation, Agarose Gel Electrophoresis
Catalog No. ABIN3188299
$61.95
100 g
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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Application:
Agarose Gel Electrophoresis
Concentration:
10 X
Catalog No. ABIN925559
$92.00
1 L
Plus shipping costs $45.00 Will be delivered in 1 to 2 Business Days
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Application:
DNA Separation, RNA Separation, Agarose Gel Electrophoresis
Catalog No. ABIN3188303
$113.40
300 tablets
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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Application:
Agarose Gel Electrophoresis
Concentration:
10 X
Catalog No. ABIN925561
$121.00
1 L
Plus shipping costs $45.00 Will be delivered in 1 to 2 Business Days
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Application:
DNA Separation, RNA Separation, Agarose Gel Electrophoresis
Catalog No. ABIN3188300
$208.95
500 g
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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Application:
DNA Separation, RNA Separation, Agarose Gel Electrophoresis
Catalog No. ABIN3188301
$403.20
2 x 500 g
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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Application:
DNA Separation, RNA Separation, Agarose Gel Electrophoresis
Catalog No. ABIN3188302
$418.95
125 g
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days