Molecular Biology Grade Agarose

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Application
DNA Separation (DNA Sep), RNA Separation (RNA Sep), Agarose Gel Electrophoresis (AGE)
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Characteristics Molecular Biology Grade agarose is the ideal multipurpose agarose for any DNA or RNA application.

Agarose specifications:
  • Nucleic acid lenght range: >1000 bp
  • Gelling temperature: 37-39 °C
  • Melting temperature: 88-90 °C
  • Gel Strengh: >1500 g/cm2
  • Dnase or Rnase activity: ND
  • DNA binding: ND
  • Eletroendosmosis: 0,1-0,2
  • Sulfate: <0,1 %
  • Purity Each lot is tested for DNase, RNase activity, melting point, gelation temperature, gel strength, electroendosmosis and absence of sulfate
    Grade Molecular Biology Grade
    Application Notes Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae. Structurally, it is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. Molecular Biology Grade agarose is ideally suited for routine analysis of DNA or RNA by gel electrophoresis and blotting.
    Comment

    As a gelling agent, agarose is used:

    1. To separate nucleic acids electrophoretically because its gels have larger pore sizes than polyacrylamide gels at low concentrations. Unlike polyacrylamide, the consistency of the gels is more solid (but also less elastic)
    2. To demonstrate cross reaction in IEP (Immuno electrophoresis) and Ouchterlony (double diffusion) plates in which antibody-antigen precipitin lines are studied
    3. To make gel plates or overlays for cells in tissue culture
    4. To form a gel matrix (either beaded and/or crosslinked), which can be used in chromatographic separations.

    Restrictions For Research Use only
    Storage RT
    Storage Comment 4°C. Do not freeze.