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SafeView™ Classic

Details for Product No. ABIN4219136, Supplier: Log in to see
Application
SDS-PAGE (SDS), Agarose Gel Electrophoresis (AGE)
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Purpose SafeView™ Classic is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.
Brand SafeView™
Specificity UV Compatible
Blue Light Compatible
Sensitivity limit: 0.1-0.3 ng DNA per band
Characteristics Easy to Use: SafeView™ Classic directly replaces EtBr. View and document your results as you would with EtBr staining. SafeView™ Classic can be viewed under UV light or blue light.
Safe: Non-carcinogenic.
Sensitive: Detect as little as 0.1 - 0.3 ng of DNA per gel band.
Unique: SafeView™ Classic is the only nucleic acid stain that can differentiate DNA and RNA in gels, by staining DNA green (max emission 520 nm) and RNA red (max emission 635 nm).
Application Notes Optimal working dilution should be determined by the investigator.
Comment

1. Prepare a 100 ml agarose or polyacrylamide solution.
2. Add 5 μl SafeViewTM Classic / SafeViewTM FireRed to the gel solution.
3. Mix gently, the solution should have no air bubbles.
4. For agarose gel, let the solution cool down to 60-70°C and cast the gel. For polyacrylamide gel, add APS and TEMED and cast the gel according to regular polyacrylamide gel casting protocol.
5. Run gel electrophoresis with 5 μl SafeViewTM Classic / SafeViewTM FireRed per 100 ml buffer.
6. View the results under UV or blue LED light.

Restrictions For Research Use only
Handling Advice Dispose SafeView™ Classic as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange, Propidium iodide).
Storage 4 °C
Storage Comment Store at 4°C for up to 2 years. Ships on blue ice.
Product cited in: Doh, Kim, Oh, Lee: "Identification and monitoring of Korean medicines derived from Cinnamomum spp. by using ITS and DNA marker." in: Genes & genomics, Vol. 39, Issue 1, pp. 101-109, 2017 (PubMed).

Dudek, Gossan, Yang, Im, Ruckshanthi, Yoshitane, Li, Jin, Wang, Boudiffa, Bellantuono, Fukada, Boot-Handford, Meng: "The chondrocyte clock gene Bmal1 controls cartilage homeostasis and integrity." in: The Journal of clinical investigation, Vol. 126, Issue 1, pp. 365-76, 2016 (PubMed).

Do, Kim, Yang, Pyo, Kim, Lee, Do, Kim, Chae, Sohn: "Decreased expression of p27 is associated with malignant transformation and extrathyroidal extension in papillary thyroid carcinoma." in: Tumour biology, Vol. 37, Issue 3, pp. 3359-64, 2016 (PubMed).

Zhao, Tsao, Lee, Tsai, Wang, Chuang, Wu, Lin: "Optical fiber sensor based on surface plasmon resonance for rapid detection of avian influenza virus subtype H6: Initial studies." in: Journal of virological methods, Vol. 233, pp. 15-22, 2016 (PubMed).

Zu, Tan, Ren, Ying: "Facile and phase-defined determination of HLA alleles with morpholino-functionalized nanoparticle probes." in: Nanomedicine : nanotechnology, biology, and medicine, 2016 (PubMed).

Mizan, Jahid, Kim, Lee, Kim, Ha et al.: "Variability in biofilm formation correlates with hydrophobicity and quorum sensing among Vibrio parahaemolyticus isolates from food contact surfaces and the distribution of the genes involved in ..." in: Biofouling, Vol. 32, Issue 4, pp. 497-509, 2016 (PubMed).

Vivero, Jaramillo, Cadavid-Restrepo, Soto, Herrera: "Structural differences in gut bacteria communities in developmental stages of natural populations of Lutzomyia evansi from Colombia's Caribbean coast." in: Parasites & vectors, Vol. 9, pp. 496, 2016 (PubMed).

Anjali, Ganga, Nadiya, Shefeek, Sabu: "Intraspecific variations in cardamom (Elettaria cardamomum Maton): assessment of genomic diversity by flow cytometry, cytological studies and ISSR analysis." in: SpringerPlus, Vol. 5, Issue 1, pp. 1560, 2016 (PubMed).