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Safe-Pack™

Details for Product No. ABIN4219138, Supplier: Log in to see
Application
SDS-PAGE (SDS), Agarose Gel Electrophoresis (AGE)
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Purpose Safe-Pack™ dyes represent a new and safe nucleic acid stains for the visualization of nucleic acids in agarose and polyacrylamide gels. These dyes eliminate the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.
Brand SafeView™
Characteristics Convenient: Safe-Pack™ dyes are provided as a 6X loading dye, and are mixed directly with samples before gel loading. Inert tracking dye is included to monitor gel progress.
Easy to Use: View and document your results as you would with EtBr staining. All dyes can be excited with UV light. In addition, Safe-Green™ can be excited with blue light. Maximum emission of Safe-Green™, Safe-Red™ and Safe-White™ are 525 nm, 630 nm and 470 nm respectively.
Safe: Non-carcinogenic.
Sensitive: Detect as little as 0.2 - 0.8 ng of DNA per gel band.
Superior: EtBr is known to cause strand breaks and nicks in DNA. Using Safe-Pack™ dyes minimize such damage, yielding higher transformation rates and lower mutation rates verses EtBr. For even better cloning results use Safe-Green™ with blue light excitation.
Components SafeView™ contains 1 mL each of Safe-Green™, Safe-Red™ and Safe-White™.
Application Notes Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.
Comment

1. Prepare a 100 ml agarose or polyacrylamide solution.
2. Mix gently without introducing any air bubbles.
3. For agarose gel, let the solution cool down to 60 - 70°C and cast the gel. For polyacrylamide gel, add APS and TEMED and cast the gel according to regular polyacrylamide gel casting protocol.
4. Mix samples and DNA marker with SafeViewTM dye at a 1:5 (dye : sample) dilution rate.
5. Following electrophoresis, view the results under UV.

Restrictions For Research Use only
Handling Advice Dispose SafeView™ as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange, Propidium iodide).
Storage 4 °C
Storage Comment Store at 4°C for up to 2 years. Ships on blue ice.