TurboNuclease

Details for Product No. ABIN2506908, Supplier: Log in to see
Species
Serratia marcescens (S. marcescens)
Host
Escherichia coli (E. coli)
Application
DNA Modification (DNA Mod)
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Purpose Pure recombinant active bacterial TurboNuclease
Specificity A nonspecific endonuclease
Characteristics TurboNuclease is a recombinant form of Serratia macescens extracellular endonuclease (encoded by the same gene of Benzonase) produced in E. coli using a proprietary process. This nonspecific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5'-phosphorylated oligonucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptional high specific activity and is free of protease activity. It is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
Alternate Names: TurboNuclease
Molecular Weight 27.0 kDa
Comment

Unit Definition: >1.3x10⁶ units/mg. One unit of TurboNuclease converts 50 μg of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl₂.

Restrictions For Research Use only
Format Liquid
Concentration 250 U/μL
Buffer 250 units/μl in a storage buffer of 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl₂ and 50 % glycerol
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Storage Comment -20°C
Expiry Date 12 months
Supplier Images
Polymerase Chain Reaction (PCR) image for TurboNuclease (ABIN2506908) TurboNuclease
SDS-PAGE (SDS) image for TurboNuclease (ABIN2506908) TurboNuclease (Image 2)