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Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74 °C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25 °C), 50mM NaCl, 5mM MgCl2, 200um each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10ug activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul. Quality Control Tests: PCR, activity, SDS-PAGE/purity, endonuclease/nickase