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Taq DNA Polymerase

Details for Product No. ABIN2781473, Supplier: Log in to see
Species
Thermus aquaticus
Host
Escherichia coli (E. coli)
Application
Polymerase Chain Reaction (PCR)
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Characteristics Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74 degree C and exhibits a half-life of 40 minutes at 95 degree C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5 ´~3 ´ direction in the presence of magnesium and also possesses a 5 ´~3 ´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
UniProt P19821
Application Notes PCR, 3 ' A-tailing of blunt ends, compatible with Vectors.
Features: Depend on an Enzyme That Works: Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions. Specify Your Own Reaction Conditions : Choose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10X Reaction Buffer containing 15mM MgCl2.Rely on a Performance-Tested Enzyme : Our PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account.
Comment

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74 °C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25 °C), 50mM NaCl, 5mM MgCl2, 200um each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10ug activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul.
Quality Control Tests: PCR, activity, SDS-PAGE/purity, endonuclease/nickase

Restrictions For Research Use only
Buffer - 10X Reaction Buffer with MgCl2 : 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP.
- 10X Reaction Buffer without MgCl2 : 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100. Include 10X Reaction Buffer without MgCl2 and separate 25mM MgCl2 Solution.Storage Buffer: Compatibility with Reaction Buffers: Taq DNA Polymerase in
- Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme. 50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100.
Storage -20 °C
Storage Comment Stable for 5 days at 10 °C, for longer period of time store at -20 °C.