T4 DNA Ligase

Details for Product No. ABIN2953733, Supplier: Log in to see
Application
Ligation (Lig)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Purpose T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with blunt or cohesive end.
Characteristics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with blunt or cohesive end. The enzyme repairs single strand nicks in duplex DNA, RNA or DNA/RNA hybrids. It has no activity on single-stranded nucleic acids. T4 DNA Ligase requires ATP as cofactor.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM Tris-Cl ( pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50 % glycerol.
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Stable for 12 months from date of receipt.