Bsu DNA Polymerase

Details for Product No. ABIN3188183, Supplier: Log in to see
Application
DNA Manipulation (DNA Man)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Characteristics Bsu DNA Polymerase, Large Fragment is from Bacillus subtilis and is modified so that it retains its 5'→ 3' DNA polymerase activity with its 5'→ 3' exonuclease domain removed. This DNA polymerase naturally lacks the 3'→ 5' exonuclease activity.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of Bsu DNA Polymerase, Large Fragment that that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37 °C.
Comment

  • Primer labelling
  • Second strand cDNA synthesis
  • DNA strand displacement synthesis

Restrictions For Research Use only
Concentration 5 U/μL
Buffer 25 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 50 mM NaCl, and 50 % (v/v) Glycerol.
Storage -20 °C