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Cas9 Nuclease Protein

Details for Product No. ABIN3188187, Supplier: Log in to see
Application
Genome Editing with Engineered Nucleases (GEEN)
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Characteristics The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. Guided by a target-specific, single guide RNA (sgRNA), the Cas9 Nuclease Protein serves to unwind the genomic DNA duplex and cleave both strands upon recognition of the target sequence by the sgRNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene.
Components Enzyme supplied with 10X Reaction Buffer
Restrictions For Research Use only
Concentration 10 μM
Buffer 10 mM Tris-HCl ( pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50 % (v/v) Glycerol.
Storage -20 °C