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DpnI

RD 20 U/μL
Catalog No. ABIN3188191
  • Application
    Restriction Digest (RD)
    Characteristics
    The DpnI restriction enzyme digests DNA at GmeATC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam+ strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60 X more slowly than fully methylated.
    Components
    Enzyme supplied with 10X Reaction Buffer
    Unit Definition
    One unit is defined as the amount of Dpn1 required to digest 1 μg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
  • Comment

    • Molecular cloning
    • Site directed mutagenesis
    • Restriction site mapping
    • Genotyping
    • Southern Blot
    • SNP
    • Restriction fragment length polymorphism (RFLP)

    Restrictions
    For Research Use only
  • Concentration
    20 U/μL
    Buffer
    10 mM Tris-HCl ( pH 7.5), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 μg/mL BSA, and 50 % (v/v) Glycerol.
    Storage
    -20 °C
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