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DpnI

Details for Product No. ABIN3188191, Supplier: Log in to see
Application
Restriction Digest (RD)
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Characteristics The DpnI restriction enzyme digests DNA at GmeATC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam+ strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60 X more slowly than fully methylated.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of Dpn1 required to digest 1 μg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Comment

  • Molecular cloning
  • Site directed mutagenesis
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • SNP
  • Restriction fragment length polymorphism (RFLP)

Restrictions For Research Use only
Concentration 20 U/μL
Buffer 10 mM Tris-HCl ( pH 7.5), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 μg/mL BSA, and 50 % (v/v) Glycerol.
Storage -20 °C