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NcoI ClearCut

RD 20000 U/mL
Catalog No. ABIN3188201
  • Application
    Restriction Digest (RD)
    Characteristics
    ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 5-15 minutes).
    Components
    NcoI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
  • Comment

    • Restriction enzyme digestion of doublestranded DNA
    • Reaction: 1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
    • Source: An E. coli strain that carries the modified NcoI gene from Nocardia carolina (ATCC 19070). The NcoI ClearCut enzyme recognizes and cuts the sequence C^CATGG.
    • Reaction Buffer: Buffer 1: 50%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
    • Methylation Sensitivity
    • dam methylation: Not Sensitive
    • dcm methylation: Not Sensitive
    • CpG Methylation: Not Sensitive

    Restrictions
    For Research Use only
  • Concentration
    20000 U/mL
    Buffer
    10 mM Tris-HCl ( pH 7.5), 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50 % (v/v) Glycerol
    Storage
    -20 °C
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