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Nt.BstNBI (Bst Nickase)

Details for Product No. ABIN3188203, Supplier: Log in to see
Application
DNA Modification (DNA Mod)
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Characteristics Nt.BstNBI (Bst Nickase) is a nicking endonuclease from Bacillus stearothermophilus that cleaves one DNA strand of a double-stranded DNA substrate. This site-specific endonuclease cleaves a single strand of DNA four base pairs away and 3' of its recognition sequence.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of Nt.BstNBI (Bst Nickase) that digests 1 μg of T7 DNA into 5 distinct bands (13, 9, 7, 6, and 4 kb) in 1 hour at 55°C in a reaction volume of 50 μl.
Comment

  • Single strand DNA nicking.

Restrictions For Research Use only
Concentration 10 U/μL
Buffer 10 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, 50 mM KCl, 200 μg/mL BSA and 50 % (v/v) Glycerol.
Storage -20 °C