RNase H, E. coli

Details for Product No. ABIN3188205, Supplier: Log in to see
Application
RNA Modification (RNA Mod)
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Characteristics RNase H (Ribonuclease H), E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. This enzyme cleaves the 3'-O-P-bond of RNA and generates 3' hydroxyl and 5' phosphate products. RNase H does not digest single-stranded or double-stranded DNA and RNA.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of RNase H, E. coli that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 37°C in 1X RNase H, E. coli Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT).
Comment

  • Removal of mRNA prior to synthesis of second strand cDNA
  • Removal of the poly(A) sequences from mRNA in the presence of oligo (dT)
  • Directed cleavage of RNA

Restrictions For Research Use only
Concentration 5 U/μL
Buffer 20 mM Tris-HCl ( pH 7.5), 100 mM KCl, 10 mM MgCl2, 0.1 mM DTT, 0.1 mM EDTA, and 50 % (v/v) Glycerol.
Storage -20 °C