RNase III, E. coli

Details for Product No. ABIN3188207, Supplier: Log in to see
Application
RNA Modification (RNA Mod)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Characteristics RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3´-overhangs with 5' phosphate and 3' hydroxyl termini.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer.
Comment

  • dsRNA digestion to short dsRNA fragments
  • Useful for RNA processing, maturation and structure studies
  • Directed cleavage of RNA
  • siRNA preparation for RNA interference in mammalian cells

Restrictions For Research Use only
Concentration 1 U/μL
Buffer 50 mM Tris-HCl ( pH 7.5), 0.5M NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1 % Triton® X-100 and 50 % (v/v) Glycerol.
Storage -20 °C