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T4 DNA Polymerase

Details for Product No. ABIN3188210, Supplier: Log in to see
Application
DNA Amplification (DNA Amp)
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Characteristics T4 DNA Polymerase catalyzes the 5'→3' synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3'→5' exonuclease activity but lacks 5'→3' exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3'-overhangs or 5'-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Comment

  • Generates blunt end DNA by filling in 5'overhangs or/and removing 3'overhangs
  • High fidelity due to strong 3'→5' exonuclease activity
  • Synthesis of labeled DNA probes by the replacement reaction
  • Sitespecific mutagenesis via primer extension from oligonucleotides

Restrictions For Research Use only
Concentration 5 U/μL
Buffer 100 mM KPO4 ( pH 6.5), 1 mM DTT, and 50 % (v/v) Glycerol.
Storage -20 °C