Uracil DNA Glycosylase

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Characteristics E. coli Uracil DNA Glycosylase (UDG) catalyses the release of free uracil via hydrolysis of the N-glycosylic bond between the uracil and sugar from uracil containing single- or double- stranded DNA. This enzyme does not show activity on oligomers of 6 or less bases or on RNA.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of Uracil DNA Glycosylase that catalyzes the release of 60 pmol of uracil per one minute from uracil-containing, double-stranded DNA at 37°C.
Comment

  • Prevents carryover contamination in PCR
  • Glycosylase mediated polymorphism detection (GMPD)
  • Sitedirected mutagenesis

Restrictions For Research Use only
Concentration 5 U/μL
Buffer 30 mM Tris-HCl ( pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.15 % Tween-20 and 50 % (v/v) Glycerol.
Storage -20 °C