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DNA Polymerase I Large (Klenow) Fragment

Details for Product No. ABIN3188231, Supplier: Log in to see
Application
DNA Amplification (DNA Amp)
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Characteristics DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coliDNA Polymerase I (abm Cat. No. G469). The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5'→3' exonuclease activity. With its inherent 3'→5' exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Comment

  • DNA blunting by fillingin 5'overhangs with unlabeled or labeled dNTPs
  • cDNA secondstrand synthesis
  • Generate singlestranded DNA probes using random primers
  • Sitedirected DNA mutagenesis using synthetic oligonucleotides
  • Dideoxy DNA sequencing of single or doublestranded DNA templates
  • 3'→5' exonuclease activity can blunt a 3'overhang

Restrictions For Research Use only
Concentration 5 U/μL
Buffer 25 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50 % (v/v) Glycerol.
Storage -20 °C