T4 Polynucleotide Kinase

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Characteristics T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-hydroxyl terminus of double and single-stranded RNA and DNA, oligonucleotides or nucleoside 3´-monophosphates. The enzyme is also capable of catalyzing the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of T4 Polynucleotide Kinase that catalyzes the incorporation of 1 nmol γ-phosphate from ATP to the 5'-hydroxyl termini of micrococcal nuclease-treated DNA in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer.
Comment

  • Labelling 5'termini of DNA or RNA to be used as:
  • primers for DNA sequencing
  • primers for PCR
  • probes for hybridization
  • probes for transcript mapping
  • markers for gel electrophoresis
  • Addition of 5'phosphates to oligonucleotides, PCR products, and DNA or RNA prior to ligation
  • Removal of 3´phosphoryl groups

Restrictions For Research Use only
Concentration 10 U/μL
Buffer 10 mM Tris-HCl ( pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 μM ATP, and 50 % (v/v) Glycerol.
Storage -20 °C