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Thermostable RNase H

Details for Product No. ABIN3188239, Supplier: Log in to see
Application
RNA Modification (RNA Mod)
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Characteristics Thermostable RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. Unlike E. coli RNase H which is inactivated at temperatures above 55 °C, Thermostable RNase H can withstand much higher temperatures. These higher temperatures allow for higher hybridization stringency for RNA-DNA heteroduplexes resulting in more specific hydrolysis of RNA. Thermostable RNase H has optimal activity above 65 °C and is active up to 95 °C making it useful for a broad range of applications.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of Thermostable RNase H that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 45°C [optimal temperature for poly(rA):poly(dT)] in 1X Thermostable RNase H Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT).
Comment

  • Highstringency hybrid selection and mapping of mRNA structure
  • Removal of mRNA prior to synthesis of second strand cDNA
  • Removal of the poly(A) sequences from mRNA in the presence of oligo (dT)
  • Directed cleavage of RNA

Restrictions For Research Use only
Concentration 5 U/μL
Buffer 50 mM Tris-HCl ( pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1 % Triton® X-100 and 50 % (v/v) Glycerol.
Storage -20 °C