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HGS Diamond Taq Polymerase

Details for Product No. ABIN3188332, Supplier: Log in to see
Application
Polymerase Chain Reaction (PCR), DNA Amplification (DNA Amp)
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Purpose HGS Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.
Brand HGS Diamond Taq®
Specificity HGS Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Characteristics HGS Diamond Taq® polymerase HGS Diamond Taq® is a chemically modified HotStart DNA polymerase, which initially lacks all activity below 74 °C. It requires a thermal activation of 10 minutes at 95 °C to reach maximal activity. This avoids non-specific priming at low temperature during the PCR set-up. HGS Diamond Taq® is more heat-stable than commonly used Taq DNA polymerase
Qualities:
  • Higher Sensitivity
  • Higher lot-to-lot consistency
    • Strickly monitored GMP and analytical processes ensuring lot-to-lot consistency
  • Ultra-low bioburden
    • Production in a GMP-Pharma facility leading to a guaranteed bioburden between 0 and 10 CFU/mL.
  • Ultra-low residual DNA content
    • QC-tested ensuring less than 1 fg (0.2 copy) of genomic E. coli DNA / Taq unit.
  • Lower risk of false positive results due to residual DNA contamination (bacterial & fungal).
Benefits:
  • Prevents non specific polymerisation primer-dimer formation and increases the PCR yield of specific products.
  • Proven performance in many applications, same enzyme as extensively validated and benchmarked HotGoldStar®.
Purity > 98 % by SDS-PAGE
Components HGS Diamond Taq® DNA Polymerase is provided at a concentration > 5 U/μl with Reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) and MgCl2 solution (25 mM MgCl2).
ProductDetails: Biological Activity Comment
  • Performance test: PCR on λ DNA - 0.5 kb fragment positive down to 5 pg
  • Performance test: PCR on genomic DNA - 0.1 kb fragment positive down to 10 pg
  • Activity 5'-3' Exonuclease - Positive
  • Ribonucleases (up to 10 U, 1 h, 37 °C): Not detectable
  • Endonucleases (up to 10 U, 16 h, 65 °C): Not detectable
  • Exonucleases (up to 10 U, 16 h, 65 °C): Not detectable
  • Nicking activity (up to 10 U, 16 h, 65 °C): Not detectable
  • Hotstart - No dectectable amplification without 95°C activation
  • E. coli residual DNA: < 1 fg / Taq Unit
Unit Definition One unit is defined as the amount of enzyme that incorporates, after activation step, 10 nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Molecular Weight MW approx. 95 kDa (SDS-PAGE)
Comment

HGS Diamond Taq® polymerase is particularly recommended for PCR applications that require ultra low levels of bacterial & fungal DNA in Diagnostic and demanding Research fields but also for amplification of templates prone to produce unspecific amplicons.

Restrictions For Research Use only
Storage -20 °C