Hot Diamond Taq DNA Polymerase

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Application
Polymerase Chain Reaction (PCR), DNA Amplification (DNA Amp)
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Purpose Hot Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.
Brand Hot Diamond Taq®
Specificity Hot Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Characteristics Hot Diamond Taq® polymerase exhibits unique Hot Start characteristics and represents a completely new "Hot Start concept". Hot start characteristics are not accomplished through chemically modification nor a blocking antibody but a proprietary agent prevents non-specific polymerization, thereby preventing primer-dimer formation and increasing the PCR yield of specific products. The enzyme needs very short activation time (100 % activated during the first PCR cycle) but is compatible with all existing protocols (from 20 sec. to 15 min. at 95 °C). Qualities:
  • Higher Sensitivity
  • Higher lot-to-lot consistency
    • Strickly monitored GMP and analytical processes ensuring lot-to-lot consistency
  • Ultra-low bioburden
    • Production in a GMP-Pharma facility leading to a guaranteed bioburden between 0 and 10 CFU/mL.
  • Ultra-low residual DNA content
    • QC-tested ensuring less than 1 fg (0.2 copy) of genomic E. coli DNA / Taq unit.
  • Lower risk of false positive results due to residual DNA contamination (bacterial & fungal).
Purity > 98 % by SDS-PAGE
Components Hot Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
  • 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
  • MgCl2 solution: 25 mM MgCl2
  • Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer, pH 8.0 (19 °C).
ProductDetails: Biological Activity Comment
  • Performance test: PCR on λ DNA - 0.5 kb fragment positive down to 5 pg
  • Performance test: PCR on genomic DNA - 0.1 kb fragment positive down to 10 pg
  • Performance test: PCR on genomic DNA - Numb - 0.3 kb fragment positive down to 10 pg
  • Activity 5'-3' Exonuclease - Positive
  • Ribonucleases (up to 10 U, 1 h, 37 °C): Not detectable
  • Endonucleases (up to 10 U, 16 h, 65 °C): Not detectable
  • Exonucleases (up to 10 U, 16 h, 65 °C): Not detectable
  • Nicking activity (up to 10 U, 16 h, 65 °C): Not detectable
  • Hotstart - No dectectable amplification without 95°C activation
  • E. coli residual DNA: < 1 fg / Taq Unit
Molecular Weight MW approx. 95 kDa (SDS-PAGE)
Comment

Hot Diamond Taq® polymerase is particularly recommended for PCR applications that require ultra low levels of bacterial & fungal DNA in Diagnostic and demanding Research fields but also for amplification of long and difficult templates.

Restrictions For Research Use only
Storage -20 °C