Taq DNA Polymerase

Details for Product No. ABIN411755, Supplier: Log in to see
Species
Thermus aquaticus
Host
Escherichia coli (E. coli)
Application
Polymerase Chain Reaction (PCR)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Purpose Taq DNA Polymerase for PCR and primer extension reactions at elevated temperature.
Characteristics Taq DNA Polymerase is a thermostable enzyme that replicates DNA and exhibits a half-life of 40 minutes at 95 °C. Taq catalyzes the polymerization of nucleotides into duplex DNA in the 5' -3' direction in the presence of magnesium. The enzyme has an apparent molecular weight of 94,000 daltons by SDS-PAGE and exhibits 5'- 3'exonuclease activity. Taq is recommended for use in PCR and primer extension reactions at elevated temperature. The 10X Reaction Buffer provided has been optimized for amplification of both short and long PCR products.
Alternate Names: DNA polymerase I, thermostable, DNA polymerase I, thermostable (EC 2.7.7.7) (Taq polymerase 1) polA pol1
Components Taq DNA Polymerase + 10X Reaction Buffer
Molecular Weight 94.0 kDa
Comment

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid insoluble material in 30 minutes at 74°C under standard DNA polymerase assay conditions.

Protocol Note: For optimal specificity and amplification rate the temperature and cycling times should be optimized for each new target or primer pair.
Restrictions For Research Use only
Format Liquid
Concentration 2.5 units/μL
Buffer 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50 % glycerol, 0.5 % Tween 20, and 0.5 % Nonidet P40.
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Storage Comment -20°C
Expiry Date 12 months