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PFU DNA Polymerase

Details for Product No. ABIN411757, Supplier: Log in to see
Species
Pyrococcus
Host
Escherichia coli (E. coli)
Application
Polymerase Chain Reaction (PCR)
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Purpose Pfu DNA Polymerase for accurate and consistent performance in PCR.
Specificity An enzyme that catalyzes the polymerization of nucleotides
Characteristics Pfu DNA Polymerase, combined with optimized buffer for high-fidelity and yield, provides the most accurate and consistent performance in PCR.enzyme demonstrates up to 20 folds greater accuracy than other similar enzyme in the market.Pfu DNA Polymerase can be used directly in any protocol requiring Pfu DNA Polymerase. The enzyme concentration and reaction buffer are already optimized for most published high-fidelity PCR protocols.
Alternate Names: DNA polymerase (EC 2.7.7.7) (Pfu polymerase), pol PF0212
Application Notes Note: For optimal specificity and amplification rate the temperature and cycling times should be optimized for each new target or primer pair.
Comment

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid insoluble material in 30 minutes at 74°C under standard DNA polymerase assay conditions.

Restrictions For Research Use only
Format Liquid
Concentration 2.5 units/μL
Buffer ENZYME STORAGE BUFFER: 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50 % glycerol, 0.5 % Tween 20, and 0.5 % Nonidet P40.
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Storage Comment -20°C
Expiry Date 12 months
Product cited in: Jin, Zhuo, Guo, Field: "p21-activated Kinase 1 (Pak1)-dependent phosphorylation of Raf-1 regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association." in: The Journal of biological chemistry, Vol. 280, Issue 26, pp. 24698-705, 2005 (PubMed).