T4 DNA Ligase

Details for Product No. ABIN411759, Supplier: Log in to see
Host
Escherichia coli (E. coli)
Application
Ligation (Lig)
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Purpose T4 DNA Ligase (5 u/ul)
Specificity An enzyme which anneals DNA strands together
Characteristics T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor.
Alternate Names: T4 DNA Ligase
Comment

Unit Definition: One unit of T4 DNA ligase is the amount of enzyme activity required to ligate EcoR I digested 1.0 μg of plasmid DNA in 5 minutes at 20°C in a 20 μl Reaction.

Restrictions For Research Use only
Format Liquid
Concentration 5 U/μL
Buffer ENZYME STORAGE BUFFER: 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 5 mM DTT, 50 % glycerol (v/v).
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months