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HotStart DNA Polymerase

Details for Product No. ABIN4219100, Supplier: Log in to see
Application
Polymerase Chain Reaction (PCR), Multiplex Assay (MA)
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Purpose Problems with non-specific amplification and primer-dimer formation? Try HotStart DNA Polymerase.
Specificity With HotStart DNA Polymerase, you can achieve
- The highest specificity with minimal background
- Superior performance
- Improved yield of desired product
Characteristics HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94 °C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, HotStart DNA Polymerase offers improved yield of desired PCR products.
Application Notes Assays with prolonged reaction setup or liquid handling:
- Multiplex PCR
- Specific amplification of difficult templates (i.e. G-C rich)
- Low copy PCR assays
- TA Cloning
Comment

250 U (50 μl)

Restrictions For Research Use only
Buffer Enzyme supplied with 10X buffer and MgSO4
Storage -20 °C
Storage Comment Store all components at -20°C.
Product cited in: Paulson, Le, Dickson, Ehlting, von Aderkas, Perlman: "Transcriptome analysis provides insight into venom evolution in a seed-parasitic wasp, Megastigmus spermotrophus." in: Insect molecular biology, Vol. 25, Issue 5, pp. 604-16, 2016 (PubMed).