2X PCR Taq Plus MasterMix with dye

Details for Product No. ABIN4219147, Supplier: Log in to see
Application
Polymerase Chain Reaction (PCR)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Purpose Streamline your PCR with 2X PCR MasterMix
Specificity The use of Taq Plus DNA Polymerase in this MasterMix offers:
- Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase
- Robust and consistent performance across a wide range of templates
- An economical alternative to Taq DNA Polymerase
Application Notes - Routine PCR amplification of DNA templates up to 6 kb
- Suitable for a wide range of PCR assays
- TA cloning
Comment

All PCR experiments should be assembled in a nuclease-free environment. In addition, DNA sample preparation, reaction set-up, and subsequent reaction(s) should be performed in separate areas to avoid cross contamination. The use of "clean" pipettors designated for PCR and aerosol resistant barrier tips are recommended. A negative control reaction (omitting DNA template) should be performed in tandem with sample PCR to confirm the absence of DNA contamination.
1. Add the following components into a sterile 0.2 ml PCR tube on ice (50 μlreaction volume).
- 2X PCR MasterMix 25 μl (Final Concentration 1X)
- DNA Template ~100 ng (Final Concentration ~2 ng/μl)
- Forward Primer (10 μM) 1-2.5 μl (Final Concentration 200-500 nM)
- Reverse Primer (10 μM) 1-2.5 μl (Final Concentration 200-500 nM)
- Nuclease-free H2O Up to 50 μl
2. Mix tube contents carefully and thoroughly, then collect all reagents by a brief centrifugation.
3. Choose an appropriate PCR amplification protocol for different DNA Polymerases.
4. Final PCR products are analyzed by agarose gel electrophoresis

Restrictions For Research Use only
Concentration 2 x
Storage 4 °C/-20 °C
Storage Comment Store at -20°C for long-term storage and at 4°C for up to two weeks.