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EasyScript™ Reverse Transcriptase

Details for Product No. ABIN4219165, Supplier: Log in to see
Application
cDNA Synthesis (cDNA)
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Purpose Synthesize cDNA from only 0.1pg of RNA with EasyScript™ Reverse Transcriptase.
Brand EasyScript™
Specificity An optimized mutational derivative of native RTase enzyme, the EasyScriptTM represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.
Characteristics Using strategic modifications, EasyScript™ Reverse Transcriptase lacks the intrinsic RNase H acitivity thereby drastically increases the yield and achievable synthesized cDNA length (up to 9kb). In addition, EasyScript™ contains a fidelity-enhancing subunit which ensures superior accuracy in reverse transcription reaction(s).
Application Notes - Synthesizing cDNA from a ssRNA
- DNA primer extension
- Sequencing dsDNA
- Constructing cDNA library
- Constructing libraries for serial analysis for gene expression (SAGE)
- Synthesizing cDNA in rapid amplification of cDNA ends (3' & 5' RACE)
- Producing template for use in RT-PCR or real-time RT-PCR
- Labelling 3'-end of duplex DNA via end-filling reactions
- Generating probes for hybridization.
Comment

100 x 20 μl reactions

Restrictions For Research Use only
Concentration 200 U/μL
Buffer Enzyme supplied with 5X RT buffer
Storage -20 °C
Storage Comment Store all components at -20°C.