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EasyScript Plus™ Reverse Transcriptase

Details for Product No. ABIN4219166, Supplier: Log in to see
Application
cDNA Synthesis (cDNA)
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Purpose Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with EasyScript™ Plus Reverse Transcriptase.
Brand EasyScript Plus™
Specificity EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Characteristics Engineered to perform under high temperatures (45 °C - 55 °C), EasyScript™ Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, EasyScript Plus™ Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.
Application Notes - Synthesizing cDNA from a ssRNA
- DNA primer extension
- Sequencing dsDNA
- Constructing cDNA library
- Constructing libraries for serial analysis for gene expression (SAGE)
- Synthesizing cDNA in rapid amplification of cDNA ends (3' & 5' RACE)
- Producing template for use in RT-PCR or real-time RT-PCR
- Labelling 3'-end of duplex DNA via end-filling reactions
- Generating probes for hybridization
Comment

100 x 20 μl reactions

Restrictions For Research Use only
Concentration 200 U/μL
Buffer Enzyme supplied with 5X RT buffer
Storage -20 °C
Storage Comment Store all components at -20°C.