EasyScript™ cDNA Synthesis SuperMix

Details for Product No. ABIN4219169, Supplier: Log in to see
Application
cDNA Synthesis (cDNA)
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Purpose Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.
Brand EasyScript™
Specificity - Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
Characteristics cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease.
Components cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Application Notes - Generation of templates for use in RT-PCR and qRT-PCR
- cDNA synthesis from ssRNA
- cDNA library construction
- Generation of probes for hybridization
- DNA primer extension
Comment

25 x 20 μl reactions

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C.
Product cited in: Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (PubMed). Method employed by authors: Luciferase Reporter Gene Assay (LRA)

Kim, Yoon, Kim, Noh, Kwon, Yi, Lee, Choi, Kang, Park, Lee, Ye: "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1- and Th17-cell-mediated immune responses." in: Biochemical pharmacology, Vol. 110-111, pp. 47-57, 2016 (PubMed).