2X PCR Bestaq™ MasterMix

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Application
Polymerase Chain Reaction (PCR)
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Purpose Streamline your PCR with 2X PCR MasterMix
Brand Bestaq™
Specificity The ready-to-use PCR MasterMix is a proprietary mixture of high quality DNA Polymerase, dNTPs, Mg2+, and PCR buffer in a 2X concentration. It contains all necessary reagents for DNA amplification. To set up a PCR, simply add DNA template, primers, and nuclease-free H2O to yield a 1X reaction mix.
The use of Bestaq™ DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Characteristics 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, we offer a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. The MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
Application Notes - High-throughput PCR
- Robust amplification of AT- and GC-Rich sequences
- RACE
- NGS Library construction
Comment

All PCR experiments should be assembled in a nuclease-free environment. In addition, DNA sample preparation, reaction set-up, and subsequent reaction(s) should be performed in separate areas to avoid cross contamination. The use of "clean" pipettors designated for PCR and aerosol resistant barrier tips are recommended. A negative control reaction (omitting DNA template) should be performed in tandem with sample PCR to confirm the absence of DNA contamination.
1. Add the following components into a sterile 0.2 ml PCR tube on ice (50 μlreaction volume).
- 2X PCR MasterMix 25 μl (Final Concentration 1X)
- DNA Template ~100 ng (Final Concentration ~2 ng/μl)
- Forward Primer (10 μM) 1-2.5 μl (Final Concentration 200-500 nM)
- Reverse Primer (10 μM) 1-2.5 μl (Final Concentration 200-500 nM)
- Nuclease-free H2O Up to 50 μl
2. Mix tube contents carefully and thoroughly, then collect all reagents by a brief centrifugation.
3. Choose an appropriate PCR amplification protocol for different DNA Polymerases.
4. Final PCR products are analyzed by agarose gel electrophoresis

Restrictions For Research Use only
Concentration 2 x
Storage 4 °C/-20 °C
Storage Comment Store at -20°C for long-term storage and at 4°C for up to two weeks.