5X All-In-One RT MasterMix

Details for Product No. ABIN4219180, Supplier: Log in to see
Application
cDNA Synthesis (cDNA)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Purpose From RNA template to cDNA in just 15 minutes completely hassle-free.
Specificity - Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Characteristics With 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience.
Application Notes - Generation of templates for use in RT-PCR and qRT-PCR
- cDNA synthesis from ssRNA
- cDNA library construction
- Generation of probes for hybridization
- DNA primer extension
Comment

25 x 10 μl reactions
In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C.
Product cited in: Deng, Gao, Liao, Cai: "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." in: Gene, Vol. 580, Issue 1, pp. 67-72, 2016 (PubMed).

Liu, Wang, Xie, Cheng, Qin, Chen, Chen: "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." in: International journal of cardiology, Vol. 228, pp. 388-393, 2016 (PubMed).

Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, pp. 189-98, 2016 (PubMed).

Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, pp. 16-25, 2016 (PubMed).

Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (PubMed).

Dai, Perez, Soria, Scarinci, Smoler, Morsucci, Suzuki, Cantero, Cantiello: "External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." in: Experimental cell research, Vol. 350, Issue 1, pp. 50-61, 2016 (PubMed).

Zhou, Chen, Wang, Jia, Zhu, Liu, Liu, Yang, Wu, Sun, Chen, Jing, Cheng: "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." in: Scientific reports, Vol. 6, pp. 25545, 2016 (PubMed).

Sun, He, Wu, Zhou, Liu, Cong, Liu: "Hypoxia inducible factor-1α-induced interleukin-33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." in: Clinical and experimental immunology, 2016 (PubMed).

Liu, Zhang, Luo, Xu, Fan, Wang: "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING-LIKE 1 (OsCOLE1) and OsCOLE1-INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." in: The New phytologist, Vol. 212, Issue 1, pp. 96-107, 2016 (PubMed).

Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (PubMed).

Smither, Pang, Brubaker: "Glucagon-Like Peptide-2 Requires a Full Complement of Bmi-1 for Its Proliferative Effects in the Murine Small Intestine." in: Endocrinology, Vol. 157, Issue 7, pp. 2660-70, 2016 (PubMed).

Zhou, Chen, Yan, Chen, Wang, Jia, Zhu, Liu, Liu, Yang, Wu, Sun, Chen, Jing, Cheng: "LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies." in: Frontiers in microbiology, Vol. 7, pp. 166, 2016 (PubMed).