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5X All-In-One RT MasterMix (with ExCellenCT Lysis Kit)

Details for Product No. ABIN4219190, Supplier: Log in to see
Application
Quantitative real-time PCR (qPCR)
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Purpose Extracting and preparing RNA templates directly from cultured cells to be reverse-transcribed into cDNA, with 5X All-In-One RT MasterMix.
Specificity The 5X All-In-One RT MasterMix is a ready-to-use formulation of all the reagents necessary for first-strand cDNA synthesis, including proprietary EasyScriptTM Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a finely balanced ratio of Oligo (dT)s and Random Primers. Coupled together, this complete system provides the ultimate convenience in generating high-quality cDNA directly from 10-105 cultured cells, suitable for a wide range of downstream applications.
Characteristics ExCellenCT Lysis Kit is an unique and effective alternative method to provide RNA templates that are extracted and processed directly from cultured cells in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template to be reverse-transcribed into cDNA using the 5X All-In-One RT MasterMix.
Components 5X All-In-One RT MasterMix
ExCellenCT Lysis Kit
Application Notes Gene expression studies
Comment

25 Preps, 100 x 20 μl reactions
This product is only intended for quick RNA extraction from cultured cells for two-steps RT-PCR or two-steps qRT-PCR gene expression analysis. This product is not suitable for typical RNA/cDNA pool preparation.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Stored all components at -20°C.