EvaGreen Express 2X qPCR MasterMix-ROX

Details for Product No. ABIN4219193, Supplier: Log in to see
Application
Quantitative real-time PCR (qPCR)
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Purpose Guaranteed high-performance real-time PCR using EvaGreen 2X qPCR MasterMix.
Characteristics - Extremely rapid activation - only 30 seconds!
- Ultra-fast qPCR thermal cycling - 10 second annealing/elongation time
- Streamlined protocol with a simple reaction set-up
- Ultra-fast quantification of a variety of gene targets
- Reduces pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments.
Application Notes - Gene-expression analysis
- Gene knockdown validation
- Microarray validation
- High throughput applications
- Virus detection and quantification
Comment

4 X 1.25 ml, for 500 reactions (20 μl)
1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles.
2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results.
3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range.
4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store at -20°C. Protect EvaGreen Express qPCR MasterMix from light.
Product cited in: Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, pp. 16-25, 2016 (PubMed).