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EvaGreen 2X qPCR MasterMix-Low ROX

Details for Product No. ABIN4219200, Supplier: Log in to see
Application
Quantitative real-time PCR (qPCR)
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Purpose Guaranteed high-performance real-time PCR using EvaGreen 2X qPCR MasterMix.
Specificity QPCR Instruments:
- ABI® 7500 Viia™
- QuantStudio
- BioGene InSyte™
- Illumina Eco
- Stratagene® Mx3000, Mx3005, Mx4000
- Analytikjena qTower Series
Characteristics - Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments
Components Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Application Notes - Gene-expression analysis
- Gene knockdown validation
- Microarray validation
- High throughput applications
- Virus detection and quantification
Comment

4 X 1.25 ml, for 500 reactions (20 μl)
1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles.
2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results.
3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range.
4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store at -20°C and protect from light. Store at 4°C if the MasterMix will be used within 2 weeks.
Product cited in: Liu, Wang, Xie, Cheng, Qin, Chen, Chen: "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." in: International journal of cardiology, Vol. 228, pp. 388-393, 2016 (PubMed).

Buragohain, Kumar, Nanda, Phulia, Mohanty, Kumar, Balhara, Ghuman, Singh, Balhara: "Serum MX2 Protein as Candidate Biomarker for Early Pregnancy Diagnosis in Buffalo." in: Reproduction in domestic animals = Zuchthygiene, Vol. 51, Issue 4, pp. 453-60, 2016 (PubMed).

Huang, Zhou, Yuan, Ge, Ran, Yang, Qiang, Li, Cui: "Proteasome Inhibitor YSY01A Enhances Cisplatin Cytotoxicity in Cisplatin-Resistant Human Ovarian Cancer Cells." in: Journal of Cancer, Vol. 7, Issue 9, pp. 1133-41, 2016 (PubMed).