TaqProbe 2X qPCR MasterMix-Low ROX

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Application
Quantitative real-time PCR (qPCR)
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Purpose Ultimate sensitivity in real-time PCR with TaqProbe 2X qPCR MasterMix.
Specificity QPCR Instruments:
- ABI® 7500 Viia™
- QuantStudio
- BioGene InSyte™
- Illumina Eco
- Stratagene® Mx3000, Mx3005, Mx4000
- Analytikjena qTower Series
Characteristics TaqProbe 2X qPCR MasterMix is designed for high throughput quantitative PCR using TaqMan® probe-based chemistry. Available with the option of ROX or fluorescein as the internal passive reference dye, TaqProbe 2X qPCR MasterMix offers superb performance in sensitivity and signal-to-noise ratio. The multiplex formulation supports quantitative amplification and detection of up to four targets simultaneously with consistent and reliable results.
- Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments
Application Notes - Gene-expression analysis
- Gene knockdown validation
- SNP genotyping assays
- CHiP
- Copy number variation
- Microarray validation
- High throughput applications
- Virus detection and quantification
Comment

4 X 1.25 ml, for 500 reactions (20 μl)
1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles.
2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results.
3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range.
4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Stored at -20°C and protect from light.