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EvaGreen 2X qPCR MasterMix-No Dye

Details for Product No. ABIN4219211, Supplier: Log in to see
Application
Quantitative real-time PCR (qPCR)
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Purpose Guaranteed high-performance real-time PCR using EvaGreen 2X qPCR MasterMix.
Specificity QPCR Instruments:
- BioRad® CFX96
- CFX384
- Chromo4™
- CFX Connect™
- Opticon 2
- MiniOpticon™
- Roche LightCycler® (2.0, 1.5, 480, 1536, Nano)
- MJ Research Opticon™
- Opticon™ 2
- Chromo® 4
- Eppendorf® Realplex 4
- BioGene SynChron™
- Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
- Eppendorf Mastercycler® realplex (s, 4 , 4s)
- Pro (S, 384)
- Nexus (gradient, eco, flat)
- Cepheid SmartCycler®
- GeneXpert
- Enigma® ML
- Idaho LightScanner® (24, 32)
- RapidCycler®2
- R.A.P.I.D (LT, LT Food)
- RAZOR EX
- JBAIDS
- Qiagen Rotor-Gene™ (Q, 6000)
- Takara Dice™
-T hermo Scientific PikoReal
- DNA-Technology DT96
- DTlite
- DT-322
- Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
- Bioneer ExicyclerTM
Characteristics - Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments
Components Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
Application Notes - Gene-expression analysis
- Gene knockdown validation
- Microarray validation
- High throughput applications
- Virus detection and quantification
Comment

4 X 1.25 ml, for 500 reactions (20 μl)
1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles.
2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results.
3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range.
4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store at -20°C and protect from light. Store at 4°C if the MasterMix will be used within 2 weeks.
Product cited in: Lee, Jeong, Kwon, Van Kien, Lee, Hong, Lee: "Drastic anthocyanin increase in response to PAP1 overexpression in fls1 knockout mutant confers enhanced osmotic stress tolerance in Arabidopsis thaliana." in: Plant cell reports, Vol. 35, Issue 11, pp. 2369-2379, 2016 (PubMed).

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Kim, Kim, Jung, Lee, Lee, Lee, Lee, You, Chung, Ye: "Potential role of 8-oxoguanine DNA glycosylase 1 as a STAT1 coactivator in endotoxin-induced inflammatory response." in: Free radical biology & medicine, Vol. 93, pp. 12-22, 2016 (PubMed).

Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, pp. 189-98, 2016 (PubMed).

Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (PubMed).

Bernaudo, Salem, Qi, Zhou, Zhang, Yang, Rosman, Deng, Ye, Yang, Vanderhyden, Wu, Peng: "Cyclin G2 inhibits epithelial-to-mesenchymal transition by disrupting Wnt/β-catenin signaling." in: Oncogene, Vol. 35, Issue 36, pp. 4816-27, 2016 (PubMed).

Lv, Liu, Zhao, Zhou, Wang, Guo, Fang: "Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid-binding protein." in: Scientific reports, Vol. 6, pp. 35751, 2016 (PubMed).

Xu, Song, Li, Tu, Liu, Liu, Wang, Wang, Cui, Liu, Shang, de Rooij, Gao, Li: "H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation." in: Nucleic acids research, Vol. 44, Issue 20, pp. 9681-9697, 2016 (PubMed).

Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (PubMed).

Smither, Pang, Brubaker: "Glucagon-Like Peptide-2 Requires a Full Complement of Bmi-1 for Its Proliferative Effects in the Murine Small Intestine." in: Endocrinology, Vol. 157, Issue 7, pp. 2660-70, 2016 (PubMed).

Bairwa, Rajapurohitam, Gan, Mangat, Proctor, Karmazyn: "Cardiomyocyte Antihypertrophic Effect of Adipose Tissue Conditioned Medium from Rats and Its Abrogation by Obesity is Mediated by the Leptin to Adiponectin Ratio." in: PLoS ONE, Vol. 11, Issue 1, pp. e0145992, 2016 (PubMed).

Nguyen, Duquette, Mamarbachi, Thorin: "Epigenetic Regulatory Effect of Exercise on Glutathione Peroxidase 1 Expression in the Skeletal Muscle of Severely Dyslipidemic Mice." in: PLoS ONE, Vol. 11, Issue 3, pp. e0151526, 2016 (PubMed).

Das, Chadwick: "Influence of Repressive Histone and DNA Methylation upon D4Z4 Transcription in Non-Myogenic Cells." in: PLoS ONE, Vol. 11, Issue 7, pp. e0160022, 2016 (PubMed).

Zhou, Wang, Ongaro, Boehm, Kaartinen, Mishina, Bernard: "Normal gonadotropin production and fertility in gonadotrope-specific Bmpr1a knockout mice." in: The Journal of endocrinology, Vol. 229, Issue 3, pp. 331-41, 2016 (PubMed).