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FlyCut™ NheI

Details for Product No. ABIN5519464, Supplier: Log in to see
Application
Restriction Digest (RD)
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Purpose Super fast restriction enzyme
Brand FlyCut™
Specificity 5'...G^CTAGC...3'
3'...CGATC^G...5'
Characteristics FlyCut® NheI is expressed and purified from E.coli that carries the recombinant NheI gene. The molecular weight is 39.1 kDa, with the recognition site at G^CTAGC. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 80oC for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Components Enzyme, 10X Buffer, 10X DNA Loading Buffer
Unit Definition One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Application Notes Genomic DNA, plasmid DNA, PCR product
Comment

Fast: 5 minutes DNA digestion.
Simple: universal buffer for all restriction enzymes.

Restrictions For Research Use only
Buffer Storage Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/mL BSA, 50 % Glycerol
10xFlyCut® Buffer: 500 mM Tris-Ac pH 7.9, 1 M KAc, 120 mM MgAc2, 1 mg/mL BSA
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months