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TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )

Details for Product No. ABIN5519499, Supplier: Log in to see
Application
cDNA Synthesis (cDNA)
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Purpose The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA.
Brand TransScript®
Specificity Simultaneous genomic DNA removal and cDNA synthesis.
The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
qPCR ready cDNA in 15 minutes.
cDNA up to 250 bp.
Characteristics It is provided at 5x concentration and used at 1x concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85oC for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR.
Components 5xTransScript® All-in-One SuperMix for PCR, 5xTransScript® All-in-One No-RT Control SuperMix for qPCR, gDNA Remover, RNase-free Water
Application Notes Multiple copy and low copy gene detection
Comment

50 rxnsx20 μl Systems

Restrictions For Research Use only
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months