TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for PCR

Details for Product No. ABIN5519506, Supplier: Log in to see
Application
cDNA Synthesis (cDNA)
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Purpose For cDNA synthesis from total RNA or mRNA with all the necessary components
Brand TransScript®
Specificity One-tube format for simple and fast setup and reduced pipetting variability.
The optimal ratio of oligo(dT)20 primer to random primer(N9) for PCR ready cDNA.
PCR ready cDNA in 30 minutes (unsuitable for qPCR).
cDNA up to 15 kb.
Characteristics TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The SuperMix is provided at 5x concentration and used at 1x concentration by adding RNA and H2O. The resulting cDNA is suitable for regular PCR, not for qPCR.
Components 5xTransScript® II All-in-One SuperMix for PCR, RNase-free Water
Application Notes cDNA library construction, 3' and 5' RACE, Multiple copy and low copy gene detection, GC-rich or complex secondary structure RNA template
Comment

50 rxnsx20 μl Systems

Restrictions For Research Use only
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months