TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for PCR
cDNA
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- Application
- cDNA Synthesis (cDNA)
- Purpose
- For cDNA synthesis from total RNA or mRNA with all the necessary components
- Brand
- TransScript®
- Specificity
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One-tube format for simple and fast setup and reduced pipetting variability.
The optimal ratio of oligo(dT)20 primer to random primer(N9) for PCR ready cDNA.
PCR ready cDNA in 30 minutes (unsuitable for qPCR).
cDNA up to 15 kb. - Characteristics
- TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The SuperMix is provided at 5x concentration and used at 1x concentration by adding RNA and H2O. The resulting cDNA is suitable for regular PCR, not for qPCR.
- Components
- 5xTransScript® II All-in-One SuperMix for PCR, RNase-free Water
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- Application Notes
- cDNA library construction, 3' and 5' RACE, Multiple copy and low copy gene detection, GC-rich or complex secondary structure RNA template
- Comment
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50 rxnsx20 μl Systems
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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