TransScript® Reverse Transcriptase

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Application
cDNA Synthesis (cDNA)
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Purpose TransScript® Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.
Brand TransScript®
Specificity Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Components TransScript® Reverse Transcriptase, 5xTS RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
Unit Definition One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
Application Notes First-strand cDNA synthesis, Multiple copy and low copy gene detection
Restrictions For Research Use only
Buffer 5xTS RT Buffer: 250 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months