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TransStart® FastPfu DNA Polymerase (with 2.5 mM dNTPs)

Details for Product No. ABIN5519530, Supplier: Log in to see
Application
Polymerase Chain Reaction (PCR)
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Purpose TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.
Brand TransStart®
Characteristics - Extension rate is about 2-4 kb/min.
- TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
- PCR products can be directly cloned into pEASY®-Blunt vectors.
- Amplification of genomic DNA fragment up to 15 kb.
- Amplification of plasmid DNA fragment up to 20 kb.
Components DNA Polymerase, 10X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Unit Definition One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Application Notes High fidelity PCR, High yield and fast PCR, Blunt end cloning, Site-directed mutagenesis, Complex templates
Comment

TransStart® FastPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Restrictions For Research Use only
Buffer Storage Buffer: 50 mM Tris-HCl ( pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50 % glycerol
5xTransStart® FastPfu Buffer with 20 mM MgSO4: 100 mM Tris-SO4 ( pH 9.2), 50 mM (NH4)2SO4, 200 mM KCl, 10 mM MgSO4, 10 % Glycerol, others
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months