TransStart® FastPfu Fly DNA Polymerase

Details for Product No. ABIN5519534, Supplier: Log in to see
Application
Polymerase Chain Reaction (PCR)
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Purpose TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase.
Brand TransStart®
Specificity TransStart® FastPfu Fly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
Characteristics - TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
- Extension rate is about 2-6 kb/min.
- PCR products can be directly cloned into pEASY®-Blunt vectors.
- Amplification of genomic DNA fragment up to 15 kb.
- Amplification of plasmid DNA fragment up to 20 kb.
Components DNA Polymerase, 5X Pfu Buffer, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Unit Definition One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Application Notes High fidelity PCR, High yield and fast PCR, Blunt end cloning, Site-directed mutagenesis, Complex templates
Comment

TransStart® FastPfu Fly DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu Fly DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Restrictions For Research Use only
Buffer Storage Buffer:50 mM Tris-HCl ( pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50 % glycerol
5xTransStart® FastPfu Fly Buffer: 100 mM Tris-SO4 pH 9.2, 50 mM (NH4)2SO4, 200 mM KCl, 10 mM MgSO4, 10 % Glycerol, others
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months
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 image for TransStart® FastPfu Fly DNA Polymerase (ABIN5519534) TransStart® FastPfu Fly DNA Polymerase
 image for TransStart® FastPfu Fly DNA Polymerase (ABIN5519534) TransStart® FastPfu Fly DNA Polymerase (Image 2)