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cDNA Synthesis

Complementary DNA (cDNA) is synthesized in a reverse transcription reaction by the enzyme reverse transcriptase from a single-stranded mRNA template. cDNA molecules are commonly used to clone and express eukaryotic genes in prokaryotes since the original genes cannot be readily cloned due to the presence of introns. Our reverse transcriptase and cDNA synthesis premixes provide you with the necessary tools for cDNA first-strand synthesis and PCR amplification specifically from mRNA or total RNA extracts.
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    cDNA Synthesis (cDNA)
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Synthesize cDNA from only 0.1pg of RNA with EasyScript™ Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
An optimized mutational derivative of native RTase enzyme, the EasyScriptTM represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.
Catalog No. ABIN4219164
$51.94
25 reactions
Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with EasyScript™ Plus Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Catalog No. ABIN4219163
$70.28
25 reactions
Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

Synthesize cDNA from only 0.1pg of RNA with EasyScript™ Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
An optimized mutational derivative of native RTase enzyme, the EasyScriptTM represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.
Catalog No. ABIN4219165
$113.06
100 reactions
Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with EasyScript™ Plus Reverse Transcriptase.

Application:
cDNA Synthesis
Concentration:
200 U/μL
Storage:
-20 °C
Specificity:
EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Catalog No. ABIN4219166
$174.17
100 reactions
Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
Publications
  • Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (Pubmed)
  • Kim, Yoon, Kim, Noh, Kwon, Yi, Lee, Choi, Kang, Park, Lee, Ye: "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1- and Th17-cell-mediated immune responses." in: Biochemical pharmacology, Vol. 110-111, Issue , pp. 47-57, 2016 (Pubmed)
Catalog No. ABIN4219169
$88.61
25 reactions
Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains EasyScript™ Plus Reverse Transcriptase.
Publications
  • Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (Pubmed)
Catalog No. ABIN4219171
$100.83
25 reactions
Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
Publications
  • Kim, Yoon, Kim, Noh, Kwon, Yi, Lee, Choi, Kang, Park, Lee, Ye: "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1- and Th17-cell-mediated immune responses." in: Biochemical pharmacology, Vol. 110-111, Issue , pp. 47-57, 2016 (Pubmed)
Catalog No. ABIN4219170
$168.06
100 reactions
Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

Convenient and time-saving reaction set-up with cDNA Synthesis SuperMix.

Application:
cDNA Synthesis
Components:
cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
Storage:
-20 °C
Specificity:
- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains EasyScript™ Plus Reverse Transcriptase.
Publications
  • Amodio, Sasso, DAmbrosio, Scaloni, Moltedo, Franceschelli, Zambrano, Remondelli: "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." in: Cell biology and toxicology, Vol. 32, Issue 4, pp. 285-303, 2016 (Pubmed)
Catalog No. ABIN4219172
$210.83
100 reactions
Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

First-Strand cDNA Synthesis SuperMix for qPCR

Application:
cDNA Synthesis
Concentration:
5 x
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Catalog No. ABIN2953682
$250.00
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

All-in-One First-Strand cDNA Synthesis SuperMix for qPCR

Application:
cDNA Synthesis
Concentration:
5 x
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Catalog No. ABIN2953687
$375.00
50 reactions
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

All-in-One First-Strand cDNA Synthesis SuperMix for PCR II

Application:
cDNA Synthesis
Concentration:
5 x
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Catalog No. ABIN2953686
$387.50
50 reactions
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

From RNA template to cDNA in just 15 minutes completely hassle-free.

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Publications
  • Deng, Gao, Liao, Cai: "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." in: Gene, Vol. 580, Issue 1, pp. 67-72, 2016 (Pubmed)
  • Liu, Wang, Xie, Cheng, Qin, Chen, Chen: "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." in: International journal of cardiology, Vol. 228, Issue , pp. 388-393, 2016 (Pubmed)
  • Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, Issue , pp. 189-98, 2016 (Pubmed)
  • Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, Issue , pp. 16-25, 2016 (Pubmed)
  • Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (Pubmed)
  • Dai, Perez, Soria, Scarinci, Smoler, Morsucci, Suzuki, Cantero, Cantiello: "External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." in: Experimental cell research, Vol. 350, Issue 1, pp. 50-61, 2016 (Pubmed)
  • Zhou, Chen, Wang, Jia, Zhu, Liu, Liu, Yang, Wu, Sun, Chen, Jing, Cheng: "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." in: Scientific reports, Vol. 6, Issue , pp. 25545, 2016 (Pubmed)
  • Sun, He, Wu, Zhou, Liu, Cong, Liu: "Hypoxia inducible factor-1α-induced interleukin-33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." in: Clinical and experimental immunology, Vol. , Issue , pp. , 2016 (Pubmed)
  • Liu, Zhang, Luo, Xu, Fan, Wang: "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING-LIKE 1 (OsCOLE1) and OsCOLE1-INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." in: The New phytologist, Vol. 212, Issue 1, pp. 96-107, 2016 (Pubmed)
  • Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (Pubmed)
  • Show more References
Catalog No. ABIN4219180
$58.06
25 reactions
Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

From RNA template to cDNA in just 15 minutes completely hassle-free.

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Publications
  • Deng, Gao, Liao, Cai: "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." in: Gene, Vol. 580, Issue 1, pp. 67-72, 2016 (Pubmed)
  • Liu, Wang, Xie, Cheng, Qin, Chen, Chen: "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." in: International journal of cardiology, Vol. 228, Issue , pp. 388-393, 2016 (Pubmed)
  • Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, Issue , pp. 189-98, 2016 (Pubmed)
  • Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, Issue , pp. 16-25, 2016 (Pubmed)
  • Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (Pubmed)
  • Dai, Perez, Soria, Scarinci, Smoler, Morsucci, Suzuki, Cantero, Cantiello: "External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." in: Experimental cell research, Vol. 350, Issue 1, pp. 50-61, 2016 (Pubmed)
  • Zhou, Chen, Wang, Jia, Zhu, Liu, Liu, Yang, Wu, Sun, Chen, Jing, Cheng: "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." in: Scientific reports, Vol. 6, Issue , pp. 25545, 2016 (Pubmed)
  • Sun, He, Wu, Zhou, Liu, Cong, Liu: "Hypoxia inducible factor-1α-induced interleukin-33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." in: Clinical and experimental immunology, Vol. , Issue , pp. , 2016 (Pubmed)
  • Liu, Zhang, Luo, Xu, Fan, Wang: "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING-LIKE 1 (OsCOLE1) and OsCOLE1-INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." in: The New phytologist, Vol. 212, Issue 1, pp. 96-107, 2016 (Pubmed)
  • Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (Pubmed)
  • Show more References
Catalog No. ABIN4219181
$131.39
100 reactions
Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

From RNA template to cDNA in just 15 minutes completely hassle-free.

Application:
cDNA Synthesis
Storage:
-20 °C
Specificity:
- Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
Publications
  • Deng, Gao, Liao, Cai: "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." in: Gene, Vol. 580, Issue 1, pp. 67-72, 2016 (Pubmed)
  • Liu, Wang, Xie, Cheng, Qin, Chen, Chen: "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." in: International journal of cardiology, Vol. 228, Issue , pp. 388-393, 2016 (Pubmed)
  • Li, Yang, Li, Jia, Li, Zhang, Xiao: "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." in: International immunopharmacology, Vol. 34, Issue , pp. 189-98, 2016 (Pubmed)
  • Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, Issue , pp. 16-25, 2016 (Pubmed)
  • Tuerdi, Xu, Zhu, Chen, Cao, Wang, Zhang, Li, Qi: "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 12, Issue 7, pp. 1899-1907, 2016 (Pubmed)
  • Dai, Perez, Soria, Scarinci, Smoler, Morsucci, Suzuki, Cantero, Cantiello: "External Ca(2+) regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." in: Experimental cell research, Vol. 350, Issue 1, pp. 50-61, 2016 (Pubmed)
  • Zhou, Chen, Wang, Jia, Zhu, Liu, Liu, Yang, Wu, Sun, Chen, Jing, Cheng: "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." in: Scientific reports, Vol. 6, Issue , pp. 25545, 2016 (Pubmed)
  • Sun, He, Wu, Zhou, Liu, Cong, Liu: "Hypoxia inducible factor-1α-induced interleukin-33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." in: Clinical and experimental immunology, Vol. , Issue , pp. , 2016 (Pubmed)
  • Liu, Zhang, Luo, Xu, Fan, Wang: "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING-LIKE 1 (OsCOLE1) and OsCOLE1-INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." in: The New phytologist, Vol. 212, Issue 1, pp. 96-107, 2016 (Pubmed)
  • Hanson, Hamilton, Perlman: "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." in: BMC evolutionary biology, Vol. 16, Issue 1, pp. 228, 2016 (Pubmed)
  • Show more References
Catalog No. ABIN4219182
$198.61
200 reactions
Will be delivered in 3 to 4 Business Days
Supplier: Log in to see

cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA.

Application:
cDNA Synthesis
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Catalog No. ABIN2953677
$200.00
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

Application:
cDNA Synthesis
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Specificity:
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
Catalog No. ABIN2953679
$225.00
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

First-Strand cDNA Synthesis SuperMix for PCR

Application:
cDNA Synthesis
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Specificity:
First-Strand cDNA Synthesis SuperMix for PCR
Catalog No. ABIN2953681
$262.50
50 reactions
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

Application:
cDNA Synthesis
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Specificity:
First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)
Catalog No. ABIN2953684
$337.50
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

Unique genomic DNA remover is combined with First-Strand cDNA Synthesis SuperMix

Application:
cDNA Synthesis
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Catalog No. ABIN2953678
$212.50
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

One-Step gDNA Removal and cDNA Synthesis SuperMix.

Application:
cDNA Synthesis
Components:
Contains: 50 tests for 20 μL Systems
Storage:
-20 °C
Catalog No. ABIN2953680
$237.50
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

Unique genomic DNA remover is combined with First-Strand cDNA Synthesis SuperMix

Application:
cDNA Synthesis
Components:
Contains: 50 reactions for 20 μL Systems
Storage:
-20 °C
Catalog No. ABIN2953685
$362.50
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

miRNA First-Strand cDNA Synthesis SuperMix

Application:
cDNA Synthesis
Components:
Contains: 20 reactions for 20 μL Systems
Storage:
-20 °C
Catalog No. ABIN2953683
$387.50
20 reactions
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity. 

Application:
cDNA Synthesis
Catalog No. ABIN2952640
$175.00
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity. 

Application:
cDNA Synthesis
Catalog No. ABIN2952641
$200.00
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability.

Application:
cDNA Synthesis
Catalog No. ABIN2952642
$312.50
10 kU
Will be delivered in 9 to 14 Business Days
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