DNA Amplification

Polymerase enzymes are responsible for replication and formation of new nucleic acid strands. Polymerase enzymes exist in many forms, and fulfill different roles in molecular biology based on template and end product:

  • DNA-dependent DNA polymerases (often referred to as DNA replication enzymes or simply DNA polymerases) recognize a template of single stranded DNA, and anneal one deoxynucleotide at a time to form a complementary strand of DNA. DNA-dependent DNA polymerases are most frequently used for DNA replication in experiments like polymerase chain reaction (PCR).
  • RNA-dependent DNA polymerases (often referred to as reverse transcriptases) recognizes a template of single stranded RNA, and anneal a single deoxynucleotide at a time to form a complete complementary strand of DNA. RNA-dependent DNA-polymerases are frequently used to generate cDNA copies of mRNA transcripts (e.g. when generating a cDNA library via RT-PCR).

More information about our Taq, Pfu and special polymerases.

11 Products
Type
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    Enzyme
Application
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Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
  • 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
  • MgCl2 solution: 25 mM MgCl2
  • Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer pH 8.0 (19 °C)
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that incorporates, 10nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Specificity:
Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188324
$103.95
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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Red Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
Red Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
  • 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
  • MgCl2 solution: 25 mM MgCl2
  • Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer pH 8.0 (19 °C)
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that incorporates, 10 nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Specificity:
Red Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188327
$103.95
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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SilverStar is a high-performance thermostable recombinant DNA polymerase, isolated from Thermus aquaticus.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
Components:
  • SilverStar DNA polymerase (100μl)
  • 10x reaction buffer (yellow cap) (2x 1.5 ml)
  • 50mM MgCl2 (2x 1.5 ml)
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C.
Specificity:
SilverStar is a high-performance thermostable recombinant DNA polymerase, isolated from Thermus aquaticus. It is an extremely robust Taq DNA polymerase that does not require optimization and gives high yields with minimal background consistently from one reaction to another. It is a highly active enzyme that produces excellent results across a wide range of applications such as DNA Array.
Catalog No. ABIN3188323
$103.09
500 U
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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Hot Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
Hot Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
  • 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
  • MgCl2 solution: 25 mM MgCl2
  • Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer, pH 8.0 (19 °C).
Storage:
-20 °C
Specificity:
Hot Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188333
$235.20
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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HGS Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
HGS Diamond Taq® DNA Polymerase is provided at a concentration > 5 U/μl with Reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) and MgCl2 solution (25 mM MgCl2).
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that incorporates, after activation step, 10 nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Specificity:
HGS Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188330
$313.60
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188193
$58.06
500 U
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Application:
DNA Amplification
Concentration:
1 U/μL
Components:
Enzyme supplied with 5X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.
Catalog No. ABIN3188236
$82.50
100 U
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188231
$82.50
500 U
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188210
$143.61
500 U
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Application:
DNA Amplification
Concentration:
10 U/μL
Components:
Enzyme supplied with 5X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
Catalog No. ABIN3188235
$204.72
1000 U
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Klenow Fragment (3'→5' Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188198
$82.50
500 U
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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