genomics-online offers you hand-selected high-quality enzymes for RNA and DNA digestion from well known manufacturers. In case you have any questions regarding our products, our customer support will gladly help you via phone, email or live chat.
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 ℃, which corresponds approximately to complete digestion of 37 μg DNA. Note that 1 KU=1000 units.
One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer.
One unit is defined as the amount of Exonuclease III, E. coli that is required to catalyze the release of 1 nmol of acid soluble nucleotides from double stranded DNA in 30 minutes at 37°C in 1X Exonuclease III Reaction Buffer.