Enzymes for PCR

The choice of a PCR enzyme can profoundly affect the outcome of the PCR as each DNA polymerase has different characteristics. In order to obtain the best results, it is important to select the polymerase best suited to the application. We offer a wide range of DNA and RNA polymerases and master mixes.

Browse our polymerases by yourself, use this detailed information table about polymerases on our site or ask our service team via mail chat or phone for guidance.

95 Products
Data Quality
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Type
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    Enzyme
Gene
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Species
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Host
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Application
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    Polymerase Chain Reaction (PCR)
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Protein Type
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Pfu DNA Polymerase for accurate and consistent performance in PCR.

Application:
Polymerase Chain Reaction
Concentration:
2.5 units/μL
Storage:
-20 °C
Specificity:
An enzyme that catalyzes the polymerization of nucleotides
Publications
  • Jin, Zhuo, Guo, Field: "p21-activated Kinase 1 (Pak1)-dependent phosphorylation of Raf-1 regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association." in: The Journal of biological chemistry, Vol. 280, Issue 26, pp. 24698-705, 2005 (Pubmed)
Catalog No. ABIN411757
$395.00
Plus shipping costs $45.00 Will be delivered in 2 to 3 Business Days
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Application:
Polymerase Chain Reaction
Storage:
-20 °C
Publications
  • Lee, Rollwagen-Bollens, Bollens, Faber-Hammond: "Environmental influence on cyanobacteria abundance and microcystin toxin production in a shallow temperate lake." in: Ecotoxicology and environmental safety, Vol. 114, Issue , pp. 318-25, 2015 (Pubmed)
  • González, Brito, González: "Identification of glycoproteins secreted by wild-type Botrytis cinerea and by protein O-mannosyltransferase mutants." in: BMC microbiology, Vol. 14, Issue , pp. 254, 2015 (Pubmed)
  • Boyd, Tennant, Melendez, Toema, Galen, Geddes, Levine: "Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of Salmonella detection in blood." in: Journal of applied microbiology, Vol. 118, Issue 5, pp. 1199-209, 2015 (Pubmed)
  • Miersch, Li, Hanna, McLaughlin, Hornsby, Matsuguchi, Paduch, Sääf, Wells, Koide, Kossiakoff, Sidhu: "Scalable high throughput selection from phage-displayed synthetic antibody libraries." in: Journal of visualized experiments : JoVE, Vol. , Issue 95, pp. 51492, 2015 (Pubmed)
  • Thekke-Veetil, Polashock, Marn, Plesko, Schilder, Keller, Martin, Tzanetakis: "Population structure of blueberry mosaic associated virus: Evidence of reassortment in geographically distinct isolates." in: Virus research, Vol. 201, Issue , pp. 79-84, 2015 (Pubmed)
  • Maxfield-Taylor, Mujic, Rao: "First detection of the larval chalkbrood disease pathogen Ascosphaera apis (Ascomycota: Eurotiomycetes: Ascosphaerales) in adult bumble bees." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124868, 2015 (Pubmed)
  • Frías, Brito, González, González: "The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif on the protein surface." in: Molecular plant pathology, Vol. 15, Issue 4, pp. 342-51, 2014 (Pubmed)
  • Acanda, Martínez, Prado, González, Rey: "EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine." in: Plant cell reports, Vol. 33, Issue 3, pp. 471-81, 2014 (Pubmed)
  • Zhang, Liu, Liu, Zhang, Cao, Ye, Wang, Zhang, Xiao, Yang, Li, Bai: "Transcriptome profiling of a multiple recurrent muscle-invasive urothelial carcinoma of the bladder by deep sequencing." in: PLoS ONE, Vol. 9, Issue 3, pp. e91466, 2014 (Pubmed)
  • Boyd, Tennant, Saague, Simon, Muhsen, Ramachandran, Cross, Galen, Pasetti, Levine: "Serum bactericidal assays to evaluate typhoidal and nontyphoidal Salmonella vaccines." in: Clinical and vaccine immunology : CVI, Vol. 21, Issue 5, pp. 712-21, 2014 (Pubmed)
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Catalog No. ABIN1536547
$60.00
Plus shipping costs $45.00 Will be delivered in 2 to 3 Business Days
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Cost-effective quick PCR with improved fidelity? With Taq Plus DNA Polymerase.

Application:
Polymerase Chain Reaction
Concentration:
5 U/μL
Storage:
-20 °C
Specificity:
With Taq Plus DNA Polymerase, you can expect
- Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase
- Robust and consistent performance across a wide range of templates
- An economical alternative to Taq DNA Polymerase
Publications
  • Xu, Cheng, Li, Wang, Zhu, Ma, Zhang, Dong, Wang, Zhang: "Identification of substituent groups and related genes involved in salecan biosynthesis in Agrobacterium sp. ZX09." in: Applied microbiology and biotechnology, Vol. 101, Issue 2, pp. 585-598, 2016 (Pubmed)
Catalog No. ABIN4219099
$58.06
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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Taq DNA Polymerase for PCR and primer extension reactions at elevated temperature.

Application:
Polymerase Chain Reaction
Concentration:
2.5 units/μL
Components:
Taq DNA Polymerase + 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN411754
$395.00
Plus shipping costs $45.00 Will be delivered in 2 to 3 Business Days
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Problems with non-specific amplification and primer-dimer formation? Try HotStart DNA Polymerase.

Application:
Polymerase Chain Reaction, Multiplex Assay
Storage:
-20 °C
Specificity:
With HotStart DNA Polymerase, you can achieve
- The highest specificity with minimal background
- Superior performance
- Improved yield of desired product
Publications
  • Paulson, Le, Dickson, Ehlting, von Aderkas, Perlman: "Transcriptome analysis provides insight into venom evolution in a seed-parasitic wasp, Megastigmus spermotrophus." in: Insect molecular biology, Vol. 25, Issue 5, pp. 604-16, 2016 (Pubmed)
Catalog No. ABIN4219098
$70.28
Plus shipping costs $45.00 Will be delivered in 3 to 4 Business Days
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The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.

Application:
Polymerase Chain Reaction
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952618
$137.50
Plus shipping costs $45.00 Will be delivered in 11 to 16 Business Days
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Application:
Polymerase Chain Reaction
Storage:
-20 °C
Publications
  • Laurin-Lemay, Angers, Benrey, Brodeur: "Inconsistent genetic structure among members of a multitrophic system: did bruchid parasitoids (Horismenus spp.) escape the effects of bean domestication?" in: Bulletin of entomological research, Vol. 103, Issue 2, pp. 182-92, 2013 (Pubmed)
Catalog No. ABIN1536548
$149.00
2500 U
Plus shipping costs $45.00 Will be delivered in 2 to 3 Business Days
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Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952628
$162.50
Plus shipping costs $45.00 Will be delivered in 11 to 16 Business Days
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Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952629
$175.00
Plus shipping costs $45.00 Will be delivered in 11 to 16 Business Days
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EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of EasyTaq® DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. It lacks 3'-5' exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.
Catalog No. ABIN5519433
$17.10
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.
Catalog No. ABIN5519422
$28.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransFast® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. It lacks 3'-5' exonuclease activity.
Catalog No. ABIN5519490
$28.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3'-5' exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransTaq®-T DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3'-5' exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.
Catalog No. ABIN5519580
$29.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3'-5' exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X HiFi Buffer I, 10X HiFi Buffer II, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Catalog No. ABIN5519574
$43.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519554
$55.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 10X Taq Buffer, 10X GC Enhancer, 6X DNA Loading Buffer
Storage:
-20 °C
Unit Definition:
One unit of TransStart® TopTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
Catalog No. ABIN5519568
$68.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Buffer, 6X DNA Loading Buffer, 50 mM MgSO2
Storage:
-20 °C
Specificity:
This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
Catalog No. ABIN5519545
$80.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
  • 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
  • MgCl2 solution: 25 mM MgCl2
  • Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer pH 8.0 (19 °C)
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that incorporates, 10nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Specificity:
Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188324
$103.95
Plus shipping costs $45.00 Will be delivered in 6 to 7 Business Days
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TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase, 5X Pfu Buffer, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
Storage:
-20 °C
Unit Definition:
One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Specificity:
TransStart® FastPfu Fly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
Catalog No. ABIN5519533
$108.00
Plus shipping costs $45.00 and $21.00 dry ice Will be delivered in 15 to 19 Business Days
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Application:
Polymerase Chain Reaction
Storage:
4 °C/-20 °C
Catalog No. ABIN2868533
$21.43
100 reactions
Plus shipping costs $45.00 Will be delivered in 9 to 11 Business Days
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