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Restriction Enzymes

Restriction enzymes (nucleases) are a class of enzymatic proteins that cut nucleotide chains at given locations or specific sequences. Restriction endonucleases are the most common form of restriction enzyme used in molecular cloning. Endonucleases recognize an internal sequence within a nucleotide chain, and cleave at that specific sequence generating either sticky ends (containing a 3’ or 5’ overhang) or blunt (containing no 3’ or 5’ overhang) ends. These ends may then be joined to a compatible end on another sequence via a DNA-ligase to prepare a desired sequence.

Restriction exonucleases do not recognize or cleave internal sequences on DNA, and instead function by removing nucleotides from a “free” or open end. Different exonucleases may work, either in a 5’ -> 3’ direction, or in a 3 -> 5’ direction. In molecular cloning, exonucleases are most commonly used to remove either a 3’ or 5’ overhang from a “sticky end” and leave, instead a “blunt” end (e.g. after a sequence has been cleaved with an endonuclease).

On genomics-online you will find a well selected variety of restriction enzymes. If you have any question regarding our products, please contact our customer support via live chat, phone, or email.

32 results :

RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188199
RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188218
RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188200
RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188194
RD 20 U/μL
Application:
Restriction Digest
Concentration:
20 U/μL
Catalog No. ABIN3188191
RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188208
RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188202
RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188201
RD 20000 U/mL
Application:
Restriction Digest
Concentration:
20000 U/mL
Catalog No. ABIN3188181
RD
Application:
Restriction Digest
Catalog No. ABIN5519446
  • Type Enzyme
    • Enzyme
  • Supplier
    • Transbionovo
    • antibodies-online
  • Application Restriction Digest (RD)
    • Restriction Digest (RD)
    • Polymerase Chain Reaction (PCR)
    • cDNA Synthesis (cDNA)
    • Genome Editing with Engineered Nucleases (GEEN)
    • Quantitative real-time PCR (qPCR)
    • DNA Modification (DNA Mod)
    • DNA Amplification (DNA Amp)
    • RNA Modification (RNA Mod)
    • Ligation (Lig)
    • DNA Manipulation (DNA Man)
  • Conjugate
    • Un-conjugated
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