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Restriction Enzymes

Restriction enzymes (nucleases) are a class of enzymatic proteins that cut nucleotide chains at given locations or specific sequences. Restriction endonucleases are the most common form of restriction enzyme used in molecular cloning. Endonucleases recognize an internal sequence within a nucleotide chain, and cleave at that specific sequence generating either sticky ends (containing a 3’ or 5’ overhang) or blunt (containing no 3’ or 5’ overhang) ends. These ends may then be joined to a compatible end on another sequence via a DNA-ligase to prepare a desired sequence.

Restriction exonucleases do not recognize or cleave internal sequences on DNA, and instead function by removing nucleotides from a “free” or open end. Different exonucleases may work, either in a 5’ -> 3’ direction, or in a 3 -> 5’ direction. In molecular cloning, exonucleases are most commonly used to remove either a 3’ or 5’ overhang from a “sticky end” and leave, instead a “blunt” end (e.g. after a sequence has been cleaved with an endonuclease).

On genomics-online you will find a well selected variety of restriction enzymes. If you have any question regarding our products, please contact our customer support via live chat, phone, or email.

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Application:
Restriction Digest
Components:
50 Units PvuRts1 I restriction enzyme
10X PvuRts1 I Reaction Buffer
1 M DTT
Storage:
-20 °C/-80 °C
Specificity:
PvuRts1 I restriction enzyme recognizes the sequence hmCN11-12/N9-10G.
Catalog No. ABIN2669403
$274.17
50 units
Will be delivered in 1 to 2 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
KpnI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188199
$58.06
20000 U
Will be delivered in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
MluI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188200
$76.39
10000 U
Will be delivered in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
NotI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188202
$76.39
10000 U
Will be delivered in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Dpn1 required to digest 1 μg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Catalog No. ABIN3188191
$106.94
2000 U
Will be delivered in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
SalI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188208
$119.17
10000 U
Will be delivered in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
AflII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188181
$192.50
10000 U
Will be delivered in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
NcoI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188201
$192.50
10000 U
Will be delivered in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
EcoRI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188194
$58.06
20000 U
Will be delivered in 3 to 4 Business Days
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Application:
Restriction Digest
Concentration:
20000 U/mL
Components:
XbaI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN3188218
$70.28
20000 U
Will be delivered in 3 to 4 Business Days
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