r-Calcitonin PCR Assay

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Gene
Application
Polymerase Chain Reaction (PCR)
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Purpose MutaGEL® r-Calcitonin test kit allows the detection of the codon changing DNA variability (C→T mutation, Pro463Leu polymorphism) in the gene of calcitonin receptor (CTR) which encodes for the corresponding protein involved in bone metabolism.
Brand MutaGEL®
Sample Type DNA
Characteristics Calcitonin receptor is a membrane protein produced by osteoclasts and it functions as transmitter of the peptide hormon calcitonin leading to decreased bone resorption processes. Sequence analyisis detected an frequently amino acid changing variability in the receptor gene. This polimorphism alters the bone density and fructure frequency if healthy persons are compared with osteoporotic patients or even between populations with different sceletal measurement. It could be shown that the polymorphism changes a phosphorylation region of the 4th intracellular domaine. By this, the in average more frequent allel “T” (Leucin) in Caucasians protects from the described pathogen effect (due to the more rare “C” allel (Prolin). Furthermore, it can be detected by specific restriction digest due to creation of a new restriction site in the gene.
Comment

MutaGEL® r-Calcitonin kit contains a set of primer for amplification of the specific sequence within the human calcitonin receptor gene CTR. Amplificates of variing genotypes (C→T mutation, Pro463Leu polymorphism) are characterized by subsequent specific restriction enzyme digestion. The variant (C, protective) posseses a restriction site for the endonuclease whereas the other variant (T, pathogen) does not. The amplified products obtained from the protective DNA-variant will therefore be cut into two fragments, whereas the pathogen DNA-variant will not be cut. The identification of the present genotype is done by analysis of present DNA-fragments by gel electrophoretic mehtods (Dr. Essrich, Biologisches Labor, Denzlingen).

Sample Volume 200 μL
Restrictions For Research Use only
Storage -20 °C