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MutaPLATE® Lactase real time PCR Kit contains specific primers and additional material for the detection of the T/C (-13910) polymorphism in the regulatory region of the lactase-phlorizin-hydrolase gene. The variable area of the regulatory region from lactase gene is amplified by PCR using genomic DNA-template. The specific primers used in the kit flank the variable area of lactase gene (LCT) and generate an amplificate of 222 bp.The standard PCR contains also sequence specific oligonucleotides marked with fluorescence dye. This specific hybridisation probe binds within the amplification product including the single nucleotide polymorphism (SNP) of target-DNA. Due to this, a fluorescence signal is generated (after excitation with 530 nm or if limited by instrument with 470 nm) and detected at 610 nm through the optical unit of the real time PCR instrument.Genotyping is performed by subsequent melting curve analysis of arised amplificates leading to unequivocal identification of C/C-genotype associated with lactose intolerance and respectively the clinical unobtrusive CT- and T/T-variants. This is due to the different melting points of the complexes formed by DNA template and “SNP-probes”. The included “SNP-probe” is 100% homologous to the C-allel. Therefore the hybridisation probe needs a higher temperature for complex-dissociation from C-allel than from the T-allel (containing a mismatch destabilizing the complex). Consequently, samples with heterozygous genotype generate both peaks at different temperatures during the melting curve analysis.