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GST-P1 PCR Assay

Details for Product No. ABIN2648749, Supplier: Log in to see
Application
Polymerase Chain Reaction (PCR)
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Purpose MutaGEL® GST-P1 PCR Assay allows the detection of the Ile105Val polymorphism in the human glutathione-S-transferase gene (GST-P1).
Brand MutaGEL®
Sample Type DNA
Characteristics The glutathione-S-transferase system protects against peroxides and electrophilic reaction partners which both can damage the cells of the body. The enzyme exists in several different isoforms. The solubility of the (manifold) substrates is increased by the addition of glutathione leading to a better elimination of the toxic metabolites from the body. Glutathione-S-transferase deficiency can be genetically determined. The GST-P1 gene compensates (at least in parts) for the frequently deletion of GST-M1 or GST-T1 genes in Caucasians (about 50% respectively 20%).
Comment

MutaGEL GST-P1 PCR Assay kit contains a set of primers which amplify a specific sequence within the human GST-P1 gene (primer GST-P1). The amplified product obtained from a wild type DNA will not be cut by the restriction enzyme included in this kit, whereas the fragment obtained from DNA carrying the Il105Val-mutation will be cut once. The identification of the present genotype is done by analysis of the amplification products and their cut fragments through gel electrophoresis.

Sample Volume 200 μL
Restrictions For Research Use only
Storage -20 °C